DNA Methyltransferase 3b in Myeloid Cells Does Not Affect the Acute Immune Response in the Airways during Pseudomonas Pneumonia.
Wanhai QinXanthe BrandsCornelis Van't VeerAlex F de VosBrendon P SciclunaTom van der PollPublished in: Cells (2022)
DNA methyltransferase 3b (Dnmt3b) has been suggested to play a role in the host immune response during bacterial infection. Neutrophils and other myeloid cells are crucial for lung defense against Pseudomonas (P.) aeruginosa infection. This study aimed to investigate the role of Dnmt3b in neutrophils and myeloid cells during acute pneumonia caused by P. aeruginosa . Neutrophil-specific ( Dnmt3b fl/fl Mrp8 Cre ) or myeloid cell-specific ( Dnmt3b fl/fl LysM Cre ) Dnmt3b-deficient mice and littermate control mice were infected with P. aeruginosa PAK via the airways. Bacteria burdens, neutrophil recruitment, and activation (CD11b expression, myeloperoxidase, and elastase levels), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) were measured in bronchoalveolar lavage fluid (BALF) at 6 and 24 h after infection. Our data showed that the bacterial loads and neutrophil recruitment and activation did not differ in BALF obtained from neutrophil-specific Dnmt3b-deficient and control mice, whilst BALF IL-6 and TNF levels were lower in the former group at 24 but not at 6 h after infection. None of the host response parameters measured differed between myeloid cell-specific Dnmt3b-deficient and control mice. In conclusion, dnmt3b deficiency in neutrophils or myeloid cells does not affect acute immune responses in the airways during Pseudomonas pneumonia.
Keyphrases
- immune response
- dna methylation
- induced apoptosis
- dendritic cells
- bone marrow
- cell cycle arrest
- acute myeloid leukemia
- respiratory failure
- liver failure
- rheumatoid arthritis
- cystic fibrosis
- endoplasmic reticulum stress
- cell death
- poor prognosis
- single cell
- stem cells
- signaling pathway
- drug induced
- intensive care unit
- toll like receptor
- metabolic syndrome
- pseudomonas aeruginosa
- hepatitis b virus
- extracorporeal membrane oxygenation
- biofilm formation
- machine learning
- cell free
- big data
- acute respiratory distress syndrome
- candida albicans
- mechanical ventilation
- circulating tumor cells