Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones.

Elie FrankMichel CailleretConstantin NelepPascal FragnerJérome PolentesElise HerardotLina El KassarKarine Giraud-TriboultChristelle MonvilleKarim Ben M'Barek

Published in: Stem cell research & therapy (2023)

This novel method of hPSC clonal isolation will greatly improve and upscale the generation of edited hPSCs required for downstream applications including disease modeling and drug screening.

Keyphrases

crispr cas
genome editing
stem cells
endothelial cells
machine learning
deep learning
high throughput
cell therapy
single cell