Calcein-Modified CeO 2 for Intracellular ROS Detection: Mechanisms of Action and Cytotoxicity Analysis In Vitro.
Nikita N ChukavinVladimir K IvanovAnton L PopovPublished in: Cells (2023)
Cerium oxide nanoparticles (CeO 2 NPs) are metal-oxide-based nanozymes with unique reactive oxygen species (ROS) scavenging abilities. Here, we studied new CeO 2 NPs modified with calcein (CeO 2 -calcein) as an intracellular ROS inactivation/visualization theranostic agent. The molecular mechanisms of the CeO 2 -calcein intracellular activity, allowing for the direct monitoring of ROS inactivation in living cells, were studied. CeO 2 -calcein was taken up by both normal (human mesenchymal stem cells, hMSc) and cancer (human osteosarcoma, MNNG/Hos cell line) cells, and was easily decomposed via endogenous or exogenous ROS, releasing brightly fluorescent calcein, which could be quantitatively detected using fluorescence microscopy. It was shown that the CeO 2 -calcein has selective cytotoxicity, inducing the death of human osteosarcoma cells and modulating the expression of key genes responsible for cell redox status as well as proliferative and migration activity. Such cerium-based theranostic agents can be used in various biomedical applications.
Keyphrases
- reactive oxygen species
- oxide nanoparticles
- living cells
- endothelial cells
- cell death
- dna damage
- induced apoptosis
- mesenchymal stem cells
- single molecule
- induced pluripotent stem cells
- fluorescent probe
- pluripotent stem cells
- photodynamic therapy
- label free
- high resolution
- cell therapy
- high throughput
- oxidative stress
- genome wide
- endoplasmic reticulum stress
- squamous cell carcinoma
- single cell
- quantum dots
- data analysis
- lymph node metastasis
- optical coherence tomography
- loop mediated isothermal amplification