Gelatin-based cell culture device for construction and X-ray irradiation of a three-dimensional oral cancer model.
Tomoka BesshoTomoko TakagiKazuyo IgawaKae SatoPublished in: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2023)
Bioassays using three-dimensional (3D) tissue models offer several advantages over 2D culture assays because they can reproduce the structure and function of native tissues. In this study, we used our newly designed gelatin device to generate a miniature 3D model of human oral squamous cell carcinoma with stroma and blood vessels. To enable air-liquid interface culture, we conceived a new device structure in which three wells were lined up and separated by a dividing thread; the wells could be connected by removing the dividing thread. Cells were seeded in the center well with the dividing thread to form a multilayer, followed by the supply of media from the side wells after thread removal. Human oral squamous cell carcinoma (HSC-4) cells, human umbilical vein endothelial cells (HUVECs), and normal human dermal fibroblasts (NHDFs) were successfully cocultured, resulting in structures that mimicked 3D-cancer tissues. This 3D-cancer model was subjected to an X-ray sensitivity assay, followed by the evaluation of DNA damage using confocal microscopy and section-scanning electron microscopy.
Keyphrases
- endothelial cells
- electron microscopy
- dna damage
- high resolution
- induced apoptosis
- gene expression
- papillary thyroid
- induced pluripotent stem cells
- cell cycle arrest
- pluripotent stem cells
- high glucose
- oxidative stress
- high throughput
- magnetic resonance imaging
- squamous cell
- signaling pathway
- cell death
- cell proliferation
- vascular endothelial growth factor
- ionic liquid
- single cell
- mass spectrometry
- tissue engineering
- bone regeneration