NanoBiT- and NanoBiT/BRET-based assays allow the analysis of binding kinetics of WNT-3A to endogenous Frizzled 7 in a colorectal cancer model.
Lukas GrätzJoanna J Sajkowska-KozielewiczJanine WesslowskiJulia KinsolvingLloyd J BridgeKatja PetzoldGary DavidsonGunnar SchultePawel KozielewiczPublished in: British journal of pharmacology (2023)
binding should be performed using receptors expressed under endogenous promotion.