Cloning, Expression, and Bioinformatics Analysis of Heavy Metal Resistance Gene afe_1862 from Acidithiobacillus ferrooxidans L1 in Escherichia coli.
Feifan LengYuanli LiWen LuoQingwei WeiYanjun JingXiaoli WangMingjun YangYong-Gang WangPublished in: Biological trace element research (2018)
Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression conditions and heterologous overexpression of afe_1862 in Escherichia coli BL21 in the presence of Cu2+ and Cd2+ were studied as well. The results indicated that AFE_1862 has higher resistance to Cu2+ than Cd2+. Bioinformatics analysis illustrated that AFE_1862 has a conserved HMA domain containing heavy metal-binding sites, which may play a role in transporting or detoxifying heavy metals.
Keyphrases
- heavy metals
- bioinformatics analysis
- escherichia coli
- binding protein
- risk assessment
- health risk assessment
- poor prognosis
- health risk
- sewage sludge
- transcription factor
- cell proliferation
- klebsiella pneumoniae
- computed tomography
- biofilm formation
- long non coding rna
- gene expression
- pet ct
- cystic fibrosis
- staphylococcus aureus
- pet imaging
- aqueous solution
- genome wide analysis
- real time pcr