Negative Modulation of the Angiogenic Cascade Induced by Allosteric Kinesin Eg5 Inhibitors in a Gastric Adenocarcinoma In Vitro Model.
Alessia RicciMarialucia GalloriniDonatella Del BufaloAmelia CataldiIlaria D'AgostinoSimone CarradoriSusi ZaraPublished in: Molecules (Basel, Switzerland) (2022)
Eg5 is a kinesin essential in bipolar spindle formation, overexpressed in tumours, thus representing a new target in cancer therapy. We aimed at evaluating the anti-cancer activity of Eg5 thiadiazoline inhibitors 2 and 41 on gastric adenocarcinoma cells (AGS), focusing on the modulation of angiogenic signalling. Docking studies confirmed a similar interaction with Eg5 to that of the parent compound K858 . Thiadiazolines were also tested in combination with Hesperidin (HSD). Cell cycle analysis reveals a reduction of G1 and S phase percentages when 41 is administered as well as HSD in combination with K858 . Western blot reveals Eg5 inhibitors capability to reduce PI3K, p-AKT/Akt and p-Erk/Erk expressions; p-Akt/Akt ratio is even more decreased in HSD+ 2 sample than the p-Erk/Erk ratio in HSD+ 41 or K858 . VEGF expression is reduced when HSD+ 2 and HSD+ 41 are administered with respect to compounds alone, after 72 h. ANGPT2 gene expression increases in cells treated with 41 and HSD+ 2 compared to K858 . The wound-healing assay highlights a reduction in the cut in HSD+ 2 sample compared to 2 and HSD. Thus, Eg5 inhibitors appear to modulate angiogenic signalling by controlling VEGF activity even better if combined with HSD. Overall, Eg5 inhibitors can represent a promising starting point to develop innovative anti-cancer strategies.
Keyphrases
- signaling pathway
- cell proliferation
- cell cycle
- induced apoptosis
- gene expression
- pi k akt
- cancer therapy
- cell cycle arrest
- endothelial cells
- dna methylation
- poor prognosis
- drug delivery
- vascular endothelial growth factor
- south africa
- radiation therapy
- high throughput
- wound healing
- rectal cancer
- locally advanced
- newly diagnosed
- structural basis