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WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat.

Dustin CramManoj KulkarniMiles BuchwaldtNandhakishore RajagopalanPankaj BhowmikKevin RozwadowskiIsobel A P ParkinAndrew G SharpeSateesh Kagale
Published in: BMC plant biology (2019)
The WheatCRISPR tool allows researchers to browse all possible gRNAs targeting a gene or sequence of interest and select effective gRNAs based on their predicted high on-target and low off-target activity scores, as well as other characteristics such as position within the targeted gene. It is publicly available at https://crispr.bioinfo.nrc.ca/WheatCrispr/ .
Keyphrases
  • genome editing
  • crispr cas
  • genome wide
  • copy number
  • cancer therapy
  • genome wide identification
  • transcription factor