WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat.
Dustin CramManoj KulkarniMiles BuchwaldtNandhakishore RajagopalanPankaj BhowmikKevin RozwadowskiIsobel A P ParkinAndrew G SharpeSateesh KagalePublished in: BMC plant biology (2019)
The WheatCRISPR tool allows researchers to browse all possible gRNAs targeting a gene or sequence of interest and select effective gRNAs based on their predicted high on-target and low off-target activity scores, as well as other characteristics such as position within the targeted gene. It is publicly available at https://crispr.bioinfo.nrc.ca/WheatCrispr/ .