The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes.
Agnieszka GęgotekSinemyiz AtalayAdelina Rogowska-WrzesińskaAgnieszka GęgotekPublished in: International journal of molecular sciences (2021)
Human epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA/B irradiated keratinocytes. The keratinocytes were cultured in a three-dimensional (3D) system, designed to mimic epidermal conditions closely. The obtained results indicate that CBD protected against the harmful effects of UVA/B radiation. CBD decreased the expression of proinflammatory proteins, including TNFα/NFκB and IκBKB complex and decreased the expression of proteins involved in de novo protein biosynthesis, which are increased in UVA/B-irradiated cells. Additionally, CBD enhanced the UV-induced expression of 20S proteasome subunits. CBD also protected protein structures from 4-hydroxynonenal (HNE)-binding induced by UV radiation, which primarily affects antioxidant enzymes. CBD-through its antioxidant/anti-inflammatory activity and regulation of protein biosynthesis and degradation-protects skin cells against UVA/B-induced changes. In the future, its long-term use in epidermal cells should be investigated.
Keyphrases
- induced apoptosis
- wound healing
- cell cycle arrest
- oxidative stress
- poor prognosis
- binding protein
- endothelial cells
- signaling pathway
- endoplasmic reticulum stress
- rheumatoid arthritis
- soft tissue
- high resolution
- radiation induced
- pi k akt
- cell death
- cell proliferation
- risk assessment
- high glucose
- mass spectrometry
- anti inflammatory
- protein protein
- inflammatory response
- diabetic rats
- lps induced
- reactive oxygen species
- current status
- life cycle
- atomic force microscopy