Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs.
Feng WanSøren S-R BohrSylvia Natalie KłodzińskaHaidar JumaaZheng HuangTommy NylanderMikkel Boas ThygesenKasper Kildegaard SørensenKnud Jørgen JensenClaus SternbergNikos S HatzakisHanne Mørck NielsenPublished in: ACS applied materials & interfaces (2019)
Inhaled antibiotic treatment of cystic fibrosis-related bacterial biofilm infections is challenging because of the pathological environment of the lungs. Here, we present an "environment-adaptive" nanoparticle composed of a solid poly lactic-co-glycolic acid (PLGA) core and a mucus-inert, enzymatically cleavable shell of d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) for the site-specific delivery of antibiotics to bacterial biofilms via aerosol administration. The hybrid nanoparticles with ultrasmall size were self-assembled via a nanoprecipitation process by using a facile microfluidic method. The interactions of the nanoparticles with the biological barriers were comprehensively investigated by using cutting-edge techniques (e.g., quartz crystal microbalance with dissipation monitoring, total internal reflection fluorescence microscopy-based particle tracking, in vitro biofilm model cultured in a flow-chamber system, and quantitative imaging analysis). Our results suggest that the mucus-inert, enzymatically cleavable TPGS shell enables the nanoparticles to penetrate through the mucus, accumulate in the deeper layer of the biofilms, and serve as sustained release depot, thereby improving the killing efficacy of azithromycin (a macrolide antibiotic) against biofilm-forming Pseudomonas aeruginosa. In conclusion, the ultrasmall TPGS-PLGA hybrid nanoparticles represent an efficient delivery system to overcome the multiple barriers and release antibiotics in a sustained manner in the vicinity of the biofilm-forming bacteria.
Keyphrases
- pseudomonas aeruginosa
- cystic fibrosis
- candida albicans
- biofilm formation
- staphylococcus aureus
- acinetobacter baumannii
- drug delivery
- high resolution
- lung function
- high throughput
- single molecule
- iron oxide
- drug release
- photodynamic therapy
- air pollution
- chronic obstructive pulmonary disease
- bone regeneration
- atomic force microscopy
- gold nanoparticles
- multidrug resistant
- quantum dots
- single cell
- optical coherence tomography
- escherichia coli
- high speed
- label free