Molluscicidal effect mechanism study on Metaldehyde to Pomacea canaliculate at low temperature.

value was only 458.8176 mg/L at 10°C, while it surged to a high of 0.8249 mg/L at 25°C. The impact of low temperature (10°C) on Metaldehyde's molluscicidal activity was analyzed via transcriptomics. The results revealed that the effect of low temperature primarily influences immunity, lipid synthesis, and oxidative stress. The expression of stress and immune-related genes, such as MANF, HSP70, Cldf7, HSP60, and PclaieFc, significantly increased. Furthermore, we studied the function of five target genes using RNAi and discovered that Cldf7 and HSP70 could notably affect Metaldehyde's molluscicidal effect. The mortality of Pomacea canaliculata increased by 36.17% (72 h) after Cldf7 interference and by 48.90% (72 h) after HSP70 interference CONCLUSION: Our findings demonstrate that low temperature can induce the extensive expression of the Cldf7 and HSP70 genes, resulting in a substantial reduction in Metaldehyde's molluscicidal activity This article is protected by copyright. All rights reserved.