Impact of Polyethylene Terephthalate Microplastics on Drosophila melanogaster Biological Profiles and Heat Shock Protein Levels.
Simran KautsYachana MishraMahendra P SinghPublished in: Biology (2024)
Microplastics and nanoplastics are abundant in the environment. Further research is necessary to examine the consequences of microplastic contamination on living species, given its widespread presence. In our research, we determined the toxic effects of PET microplastics on Drosophila melanogaster at the cellular and genetic levels. Our study revealed severe cytotoxicity in the midgut of larvae and the induction of oxidative stress after 24 and 48 h of treatment, as indicated by the total protein, Cu-Zn SOD , CAT , and MDA contents. For the first time, cell damage in the reproductive parts of the ovaries of female flies, as well as in the accessory glands and testes of male flies, has been observed. Furthermore, a decline in reproductive health was noted, resulting in decreased fertility among the flies. By analyzing stress-related genes such as hsp 83, hsp 70, hsp 60, and hsp 26, we detected elevated expression of hsp83 and hsp70 . Our study identified hsp83 as a specific biomarker for detecting early redox changes in cells caused by PET microplastics in all the treated groups, helping to elucidate the primary defense mechanism against PET microplastic toxicity. This study offers foundational insights into the emerging environmental threats posed by microplastics, revealing discernible alterations at the genetic level.
Keyphrases
- heat shock protein
- drosophila melanogaster
- heat shock
- heat stress
- oxidative stress
- human health
- computed tomography
- stem cells
- risk assessment
- positron emission tomography
- induced apoptosis
- gene expression
- poor prognosis
- dna damage
- early onset
- pet imaging
- cell death
- young adults
- replacement therapy
- bone marrow
- ischemia reperfusion injury
- genome wide
- newly diagnosed
- endoplasmic reticulum stress
- infectious diseases