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Impact of Lipid Ratio on the Permeability of Mixed Phosphatidylcholine/Phosphatidylglycerol Membranes in the Presence of 1-Dodecyl-3-methylimidazolium Bromide Ionic Liquid.

Sandeep KumarMarkus FischerNavleen KaurHolger A ScheidtVenus Singh Mithu
Published in: The journal of physical chemistry. B (2021)
We have studied the impact of the lipid ratio on the membrane permeability of mixed phosphatidylcholine (POPC)/phosphatidylglycerol (POPG) membranes induced by 1-dodecyl-3-methylimidazolium bromide ([C 12 MIM] + Br - ) ionic liquid by evaluating the role of affinity and architecture of the phospholipid bilayer. Nine different model membranes composed of negatively charged POPG and zwitterionic POPC lipids mixed in molar ratios of 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, and 1:9 have been studied. The membrane permeability of each composition has been evaluated using fluorescence-based dye leakage assays. Despite having the highest membrane affinity, POPG-rich membranes doped with 10 and 20 mol % POPC are found to be the least permeable. 31 P- and 2 H-based solid-state NMR investigations reveal that the minor POPC component is homogeneously dispersed in the PG/PC (8:2) membrane. In contrast, the lipids seem to be segregated into POPG- and POPC-rich domains in the complementary PG/PC (2:8) composition. Although [C 12 MIM] + cations have a stronger interaction with the POPG component in the mixed membranes, their insertion has a limited impact on the overall structure and dynamics of the PG/PC (8:2) composition.
Keyphrases
  • ionic liquid
  • solid state
  • room temperature
  • fatty acid
  • endothelial cells
  • magnetic resonance
  • highly efficient
  • single molecule
  • computed tomography
  • high throughput
  • mass spectrometry
  • gene expression
  • dna methylation