Possible detrimental effects of beta-2-microglobulin knockout in pigs.

Hendrik Johannes SakeAntje FrenzelAndrea Lucas-HahnMonika Nowak-ImialekPetra HasselKlaus-Gerd HadelerDoris HermannRoswitha BeckerHeinke EylersRabea HeinWiebke BaarsAntje BrinkmannReinhard SchwinzerHeiner NiemannBjoern Petersen

Published in: Xenotransplantation (2019)

Here, we demonstrate the feasibility of generating SLA class I knockout pigs by targeting the porcine beta-2-microglobulin gene using the CRISPR/Cas9 system. Additionally, our findings indicate for the first time that this genetic modification might have a negative impact on the viability of the animals. These issues need to be solved to unveil the real value for xenotransplantation in the future.

Keyphrases

crispr cas
genome wide
genome editing
copy number
current status
gene expression
wild type