Fine mapping of the reduced height gene Rht22 in tetraploid wheat landrace Jianyangailanmai (Triticum turgidum L.).
Chao WangYunjing BaoQin YaoDan LongXue XiaoXing FanHouyang KangJian ZengLina ShaHaiqin ZhangDandan WuYonghong ZhouQiang ZhouYi WangYiran ChengPublished in: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik (2022)
RIL_DJ population, which was derived from a cross between dwarf Polish wheat (T. polonicum L., 2n = 4x = 28, AABB) and JAM. Two plant height loci, Qph.sicau-4B and Qph.sicau-7A, were mapped on chromosomes 4BS and 7AS, respectively. Qph.sicau-7A was mapped to the 0.33-4.46 Mb interval on 7AS and likely represents the candidate region of Rht22. Fine mapping confirmed and narrowed Rht22 on chromosome arm 7AS between Xbag295.s53 and Xb295.191 in three different populations. The physical region ranged from 0.53 to 1.48 Mb and included 18 candidate genes. Transcriptome analysis of two pairs of near-isogenic lines revealed that 135 differentially expressed genes (DEGs) were associated with semi-dwarfism. Of these, the expression of 83 annotated DEGs involved in hormones synthesis and signal transduction, cell wall composition, DNA replication, microtubule and phragmoplast arrays was significantly down-regulated in the semi-dwarf line. Therefore, Rht22 causes semi-dwarfism in JAM by disrupting these cellular processes, which impairs cell proliferation and reduces internode cell number.
Keyphrases
- cell wall
- genome wide
- single cell
- genome wide identification
- cell proliferation
- body mass index
- high density
- copy number
- high resolution
- air pollution
- dna methylation
- transcription factor
- poor prognosis
- rna seq
- physical activity
- mental health
- cell cycle
- binding protein
- genome wide analysis
- mass spectrometry
- bioinformatics analysis