Cryopreservation of human mucosal tissues.
Sean M HughesApril L FerreSarah E YanduraCory ShetlerChris A R BakerFernanda L CalienesClaire N LevyRena D AstronomoZhiquan ShuGretchen M LentzMichael FialkowAnna C KirbyM Juliana McElrathElizabeth SinclairLisa C RohanPeter L AndersonBarbara L ShacklettCharlene S DezzuttiDayong GaoFlorian HladikPublished in: PloS one (2018)
Cryopreservation of intact cervicovaginal and colorectal tissues with dimethylsulfoxide works well in a range of assays, while the utility of vitrification is more limited. Cell yields are higher from cryopreserved intact tissue pieces than from thawed cryopreserved single cell suspensions isolated before freezing, but T cell functions are similar.