Single-cell profiling of pediatric T-cell acute lymphoblastic leukemia: Impact of PTEN exon 7 mutation on PI3K/Akt and JAK-STAT signaling pathways.
Paola BonaccorsoCristina BugarinChiara BuracchiGrazia FazioAndrea BiondiLuca Lo NigroGiuseppe GaipaPublished in: Cytometry. Part B, Clinical cytometry (2020)
Phosphoflow analysis represents a fast, reliable, and accurate method to study the signaling profile of T-ALL. PTEN exon 7 mutated T-ALL cells are nonresponsive to IL7 in vitro suggesting that they may activate other mechanisms to support their viability and proliferation such as a higher constitutive PI3K/Akt signaling. Further investigations are necessary to elucidate the significance of this peculiar signaling behavior. Our observations should be taken into account in future studies aiming at molecular targeting of PI3K and/or JAK/STAT pathways for pharmacological intervention in T-ALL.
Keyphrases
- pi k akt
- signaling pathway
- cell cycle arrest
- induced apoptosis
- cell proliferation
- single cell
- acute lymphoblastic leukemia
- epithelial mesenchymal transition
- randomized controlled trial
- rna seq
- high throughput
- high resolution
- drug delivery
- cell death
- cancer therapy
- current status
- allogeneic hematopoietic stem cell transplantation
- endoplasmic reticulum stress
- mass spectrometry