SalivaDirect: an alternative to a conventional RNA extraction protocol for molecular detection of SARS-CoV-2 in a clinical setting.
Mohammad Khaja Mafij UddinMohammad Enayet HossainJenifar Quaiyum AmiRashedul HasanMd Mahmudul HasanAshabul IslamMd Jahid HasanNusrat Jahan ShalyShahriar AhmedPushpita SaminaMohammed Ziaur RahmanMustafizur RahmanSayera BanuPublished in: Microbiology spectrum (2023)
Affordable and accessible tests for COVID-19 allow for timely disease treatment and pandemic management. SalivaDirect is a faster and easier method to implement than NPS sampling. Patients can self-collect saliva samples at home or in other non-clinical settings without the help of a healthcare professional. Sample processing in SalivaDirect is less complex and more adaptable than in conventional nucleic acid extraction methods. We found that SalivaDirect has good diagnostic performance and is ideal for large-scale testing in settings where supplies may be limited or trained healthcare professionals are unavailable.
Keyphrases
- sars cov
- nucleic acid
- coronavirus disease
- healthcare
- end stage renal disease
- respiratory syndrome coronavirus
- chronic kidney disease
- newly diagnosed
- ejection fraction
- randomized controlled trial
- prognostic factors
- peritoneal dialysis
- patient reported outcomes
- replacement therapy
- social media
- high intensity
- patient reported