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Biosynthesis of Polyhydroxyalkanoates in Cupriavidus necator B-10646 on Saturated Fatty Acids.

Natalia O ZhilaKristina Yu SapozhnikovaEvgeniy G KiselevEkaterina I ShishatskayaTatiana G Volova
Published in: Polymers (2024)
It has been established that the wild-type Cupriavidus necator B-10646 strain uses saturated fatty acids (SFAs) for growth and polyhydroxyalkanoate (PHA) synthesis. It uses lauric (12:0), myristic (14:0), palmitic (16:0) and stearic (18:0) acids as carbon sources; moreover, the elongation of the C-chain negatively affects the biomass and PHA yields. When bacteria grow on C12 and C14 fatty acids, the total biomass and PHA yields are comparable up to 7.5 g/L and 75%, respectively, which twice exceed the values that occur on longer C16 and C18 acids. Regardless of the type of SFAs, bacteria synthesize poly(3-hydroxybutyrate), which have a reduced crystallinity (C x from 40 to 57%) and a molecular weight typical for poly(3-hydroxybutyrate) (P(3HB)) (M w from 289 to 465 kDa), and obtained polymer samples demonstrate melting and degradation temperatures with a gap of about 100 °C. The ability of bacteria to assimilate SFAs opens up the possibility of attracting the synthesis of PHAs on complex fat-containing substrates, including waste.
Keyphrases
  • fatty acid
  • wild type
  • wastewater treatment
  • high resolution
  • anaerobic digestion
  • heavy metals
  • drinking water
  • sewage sludge