Protective Effect of Orbitides from Linseed (Linum usitatissimum L.) against Ultraviolet B-induced Photoaging in Zebrafish.

Excessive ultraviolet (UV) exposure is the primary environmental factor that contributes to skin aging. Orbitides have been considered as important functional components in linseed and they are complex and multiple. In this study, linseed orbitides were divided into oxidized linseed orbitides (OLOs) and reduced linseed orbitides (RLOs), and used to investigate protection against Ultraviolet B-induced photoaging in zebrafish. Firstly, the results of the zebrafish embryo acute toxicity test showed that the OLOs had obvious embryo toxicity compared with RLOs. And RLOs had better effect in Ultraviolet B-treated zebrafish, which may significantly reduce the ROS levels and inhibit the degradation of collagen. Besides, we also found that RLOs could effectively inhibit the oxidation of proteins and lipids and regulate the activity of antioxidant enzymes. Furthermore, neutrophils recruitment to the dorsal and caudal fin regions was observed in UVB-treated zebrafish, and RLOs effectively alleviated this migration. In conclusion, RLOs has strong photoprotective effects and potential to be used as antiphotoaging ingredients.