The Antiapoptosis Effect of Geum japonicum Thunb. var. chinense Extracts on Cerebral Ischemia Reperfusion Injury via PI3K/Akt Pathway.
Bingjin OuWei TaoSongbai YangJiateng FengJinfeng WangTong YangHanyu WuYaguang HuangLingjing TanWeifeng HuangZhitao FengZhi-Gang MeiPublished in: Evidence-based complementary and alternative medicine : eCAM (2018)
Geum japonicum Thunb. var. chinense (GJ) is a type of wild vegetable found in China and other Asian countries; it has been reported that its extracts possess a neuroprotective effect against cerebral ischemia reperfusion (CIR) injury. The aim of this study is to explore the effect GJ extracts on transient focal CIR injury and neurons apoptosis and to clarify its possible underlying mechanisms in vivo. Our results indicated that pretreatment with GJ extracts significantly ameliorated the infarct volume, decreased neurological deficits, lessened neural cells apoptosis, downregulated GFAP activity level, and increased surviving neurons. Moreover, GJ extracts preadministration increased Bcl-2 levels and attenuated the increase in the expressions of Bax and it also lowered the cleaved caspase-3 activity in ischemic cortex tissues which was caused by CIR and increased the expression of PI3K and p-Akt. The above effects of high dose of GJ (GJ-H) group were much better than those of low dose of GJ (GJ-L), which indicated that GJ extracts may be helpful in the suppression of CIR injury with a dose-dependent manner.
Keyphrases
- low dose
- induced apoptosis
- high dose
- cerebral ischemia
- cell cycle arrest
- oxidative stress
- ischemia reperfusion injury
- endoplasmic reticulum stress
- cell death
- subarachnoid hemorrhage
- spinal cord
- signaling pathway
- poor prognosis
- traumatic brain injury
- gene expression
- cell proliferation
- blood brain barrier
- brain injury
- coronary artery disease
- left ventricular
- long non coding rna
- binding protein