Piperine Reduces Neoplastic Progression in Cervical Cancer Cells by Downregulating the Cyclooxygenase 2 Pathway.
Luana Pereira CardosoStefanie Oliveira de SousaJuliana Prado Gusson-ZanetoniLaura Luciana de Melo Moreira SilvaBarbara Maria FrigieriTiago HenriqueEloiza Helena TajaraSonia Maria OlianiFlávia Cristina Rodrigues-LisoniPublished in: Pharmaceuticals (Basel, Switzerland) (2023)
Cervical cancer is the fourth-most common type of cancer in the world that causes death in women. It is mainly caused by persistent infection by human papillomavirus (HPV) that triggers a chronic inflammatory process. Therefore, the use of anti-inflammatory drugs is a potential treatment option. The effects of piperine, an amino alkaloid derived from Piper nigrum , are poorly understood in cervical cancer inflammation, making it a target of research. This work aimed to investigate the antitumor effect of piperine on cervical cancer and to determine whether this effect is modulated by the cyclooxygenase 2 (PTGS2) pathway using in vitro model of cervical cancer (HeLa, SiHa, CaSki), and non-tumoral (HaCaT) cell lines. The results showed that piperine reduces in vitro parameters associated with neoplastic evolution such as proliferation, viability and migration by cell cycle arrest in the G1/G0 and G2/M phases, with subsequent induction of apoptosis. This action was modulated by downregulation of cyclooxygenase 2 (PTGS2) pathway, which in turn regulates the secretion of cytokines and the expression of mitogen-activated protein kinases (MAPKs), metalloproteinases (MMPs), and their antagonists (TIMPs). These findings indicate the phytotherapeutic potential of piperine as complementary treatment in cervical cancer.
Keyphrases
- cell cycle arrest
- cell death
- oxidative stress
- pi k akt
- signaling pathway
- anti inflammatory drugs
- poor prognosis
- combination therapy
- polycystic ovary syndrome
- type diabetes
- squamous cell carcinoma
- nitric oxide synthase
- pregnant women
- young adults
- squamous cell
- fluorescent probe
- human health
- essential oil
- pregnancy outcomes