A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots.
Thibault MounierSergi Navarro-SanzCharlotte BureauLefeuvre AntoineFabrice VaroquauxFranz DurandetChristophe PérinPublished in: BMC molecular and cell biology (2020)
The detailed protocol and the critical steps required to optimize its use for other species will democratize tissue-specific transcriptome approaches combining LM with ddRT-PCR for analyses of plants.