Bioinspired Platelet-Anchored Electrospun Meshes for Tight Inflammation Manipulation and Chronic Diabetic Wound Healing.

Tight manipulation of the initial leukocytes infiltration and macrophages plasticity towards the M2 phenotype remain a challenge for diabetic wound healing. Inspired by the platelet function and platelet-macrophage interaction, a platelet-anchored polylactic acid-b- polyethylene glycol-b-polylactic acid (PLA-PEG-PLA) electrospun dressing was developed for inflammatory modulation and diabetic wounds healing acceleration. PLA-PEG-PLA electrospun meshes encapsulated with thymosin β4 (Tβ4) and CaCl 2 was fabricated with electrospinning, followed by immersion of electrospun mesh in platelet-rich plasma to firmly anchor the platelets. We demonstrated that the anchored platelets on electrospun mesh could enhance the initial macrophage recruitment and control the Tβ4 release from electrospun meshes to facilitate the macrophages polarization to the M2 phenotype. The inflammatory regulation promoted the expression of vascular endothelial growth factor (VEGF) and the migration of vascular endothelial cells for angiogenesis, resulting in accelerated diabetic wounds healing. Therefore, our work paved a new way to design platelet-inspired electrospun meshes for inflammation manipulation and diabetic wound healing. This article is protected by copyright. All rights reserved.