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Structure and assembly of a bacterial gasdermin pore.

Alex G JohnsonMegan L MayerStefan L SchäferNora K McNamara-BordewickGerhard HummerPhilip J Kranzusch
Published in: bioRxiv : the preprint server for biology (2023)
In response to pathogen infection, gasdermin (GSDM) proteins form membrane pores that induce a host cell death process called pyroptosis 1-3 . Studies of human and mouse GSDM pores reveal the functions and architectures of 24-33 protomers assemblies 4-9 , but the mechanism and evolutionary origin of membrane targeting and GSDM pore formation remain unknown. Here we determine a structure of a bacterial GSDM (bGSDM) pore and define a conserved mechanism of pore assembly. Engineering a panel of bGSDMs for site-specific proteolytic activation, we demonstrate that diverse bGSDMs form distinct pore sizes that range from smaller mammalian-like assemblies to exceptionally large pores containing >50 protomers. We determine a 3.3 Å cryo-EM structure of a Vitiosangium bGSDM in an active slinky-like oligomeric conformation and analyze bGSDM pores in a native lipid environment to create an atomic-level model of a full 52-mer bGSDM pore. Combining our structural analysis with molecular dynamics simulations and cellular assays, we define a stepwise model of GSDM pore assembly and demonstrate that pore formation is driven by local unfolding of membrane-spanning β-strand regions and pre-insertion of a covalently bound palmitoyl into the target membrane. These results yield insights into the diversity of GSDM pores found in nature and the function of an ancient post-translational modification in enabling a programmed host cell death process.
Keyphrases
  • cell death
  • molecular dynamics simulations
  • endothelial cells
  • genome wide
  • dna methylation
  • molecular docking
  • transcription factor
  • high throughput
  • fatty acid
  • nlrp inflammasome