CRISPR-Cas12a trans-cleaves DNA G-quadruplexes.

Ying LiTao LiBi-Feng LiuRui HuJiang ZhuTing HeXin Zhou Conggang LiYunhuang YangMaili Liu

Published in: Chemical communications (Cambridge, England) (2021)

We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.

Keyphrases

crispr cas
genome editing
single molecule
circulating tumor
endothelial cells
cell free
magnetic resonance
atomic force microscopy
high resolution
living cells
fluorescent probe
nucleic acid
dna binding
transcription factor
nk cells