The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol.
Agnese GugliandoloSerena SilvestroLuigi ChiricostaFederica PollastroPlacido BramantiEmanuela MazzonPublished in: Life (Basel, Switzerland) (2020)
More than 120 cannabinoids were isolated from Cannabis sativa. In particular, Cannabidiol (CBD) and Cannabigerol (CBG) represent the two most studied non-psychoactive cannabinoids. However, CBG is less studied and less data are available on its biological properties and influence on synaptic transmission. On the contrary, CBD is already known to modulate brain excitatory glutamate, inhibitory γ-aminobutyric acid (GABA) and dopamine neurotransmission. In this study, using Next-Generation Sequencing (NGS) technology, we evaluated how CBG (1 or 5 µM) and CBD (1 or 5 µM) influence the transcriptome of the main neurotransmission pathways in NSC-34 motor neuron-like cells. At first, we evaluated that CBG and CBD were not cytotoxic and decreased the expression of pro-apoptotic genes. CBG and CBD are able to influence the expression of the genes involved in glutamate, GABA and dopamine signaling. Interestingly, the transcriptional changes induced by CBG were similar compared to CBD.
Keyphrases
- poor prognosis
- prefrontal cortex
- gene expression
- genome wide
- cell death
- uric acid
- binding protein
- anti inflammatory
- oxidative stress
- transcription factor
- multiple sclerosis
- resting state
- white matter
- long non coding rna
- single cell
- functional connectivity
- copy number
- blood brain barrier
- brain injury
- solid state
- circulating tumor
- genome wide identification
- cell free