Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants.
Simon Sau Yin LawMasato AsanumaJingwen ShouYasuyuki OzekiYutaka KodamaKeiji NumataPublished in: JACS Au (2023)
Plants can rapidly respond to different stresses by activating multiple signaling and defense pathways. The ability to directly visualize and quantify these pathways in real time using bioorthogonal probes would have practical applications, including characterizing plant responses to both abiotic and biotic stress. Fluorescence-based labels are widely used for tagging of small biomolecules but are relatively bulky and with potential effects on their endogenous localization and metabolism. This work describes the use of deuterium- and alkyne-derived fatty acid Raman probes to visualize and track the real-time response of plants to abiotic stress within the roots. Relative quantification of the respective signals could be used to track their localization and overall real-time responses in their fatty acid pools due to drought and heat stress without labor-intensive isolation procedures. Their overall usability and low toxicity suggest that Raman probes have great untapped potential in the field of plant bioengineering.
Keyphrases
- heat stress
- fatty acid
- single molecule
- fluorescence imaging
- small molecule
- living cells
- high resolution
- heat shock
- nucleic acid
- arabidopsis thaliana
- fluorescent probe
- photodynamic therapy
- climate change
- raman spectroscopy
- label free
- healthcare
- oxidative stress
- stress induced
- mass spectrometry
- genome wide identification
- quantum dots
- transcription factor
- innate immune