An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster.

Arnau FiolFederico Jurado-RuizElena López-GironaMaría José Aranzana

Published in: Plant methods (2022)

CRISPR-Cas9 enrichment is a versatile and powerful tool for long-read targeted sequencing even on highly duplicated and/or polymorphic genomic regions, being especially useful when a reference genome is not available. Potential uses of this methodology as well as its limitations are further discussed.

Keyphrases

crispr cas
genome editing
single cell
genome wide
copy number
transcription factor
cancer therapy
single molecule
dna methylation
human health
gene expression
drug delivery
risk assessment
climate change