Less Virulent Leptosphaeria biglobosa Immunizes the Canola Plant to Resist Highly Virulent L. maculans, the Blackleg Pathogen.
Kaluhannadige Rasanie Eranka PadmathilakeWannakuwattewaduge Gerard Dilantha FernandoPublished in: Plants (Basel, Switzerland) (2022)
Leptosphaeria biglobosa is a less virulent Leptosphaeria spp. that causes blackleg disease in canola. Previous studies from our lab have shown that inoculation with the less virulent L. biglobosa can boost the resistance of canola plants against the highly virulent L. maculans . The objective of this study was to confirm the effectiveness of L. biglobosa as a biocontrol agent against L. maculans utilizing morphology, fluorescence microscopy, gene quantification, and transcriptomic analysis. The in planta development of two Leptosphaeria species inoculated at different time points was assessed using fluorescent protein-tagged isolates which are GFP-tagged L. maculans and DsRed-tagged L. biglobosa . The growth inhibition of L. maculans by pre-and co-inoculated L. biglobosa was supported by no lesion development on cotyledons and no or weak fluorescence protein-tagged mycelia under the confocal microscope. The host defense-related genes, WRKY33 , PR1 , APX6 , and CHI, were upregulated in L. biglobosa inoculated Westar cotyledons compared to L. maculans inoculated cotyledons. The quantification of each pathogen through qPCR assay and gene expressions analysis on host defense-related genes by RT-qPCR confirmed the potential of L. biglobosa "brassicae' in the management of the blackleg disease pathogen, L. maculans 'brassicae', in canola.
Keyphrases
- single molecule
- candida albicans
- high throughput
- randomized controlled trial
- copy number
- genome wide identification
- disease virus
- genome wide
- high resolution
- transcription factor
- protein protein
- gene expression
- genetic diversity
- living cells
- dna methylation
- mass spectrometry
- climate change
- single cell
- high speed
- human health
- case control
- genome wide analysis