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HLA-B*27 typing using a triplex real time PCR in routine laboratory.

Marte K VikenAlice L PedersenMenaka AndersenTore JensenBenedicte A LieLine M L Boulland
Published in: HLA (2021)
We have established an in-house HLA-B*27 multiplex typing assay by using a combination of previously published, newly designed and commercial primers and probes for use with real-time PCR instruments. Hence, facilitating quick and large-scale HLA-B*27 typing.
Keyphrases
  • real time pcr
  • genetic diversity
  • small molecule
  • high throughput
  • fluorescence imaging
  • single molecule
  • patient reported outcomes
  • randomized controlled trial
  • living cells