HLA-B*27 typing using a triplex real time PCR in routine laboratory.

Marte K VikenAlice L PedersenMenaka AndersenTore JensenBenedicte A LieLine M L Boulland

Published in: HLA (2021)

We have established an in-house HLA-B*27 multiplex typing assay by using a combination of previously published, newly designed and commercial primers and probes for use with real-time PCR instruments. Hence, facilitating quick and large-scale HLA-B*27 typing.

Keyphrases

real time pcr
genetic diversity
small molecule
high throughput
fluorescence imaging
single molecule
patient reported outcomes
randomized controlled trial
living cells