scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation.
E MadissoonA Wilbrey-ClarkR J MiragaiaK Saeb-ParsyK T MahbubaniN GeorgakopoulosP HardingK PolanskiN HuangK Nowicki-OsuchR C FitzgeraldK W LoudonJ R FerdinandM R ClatworthyA TsingeneS van DongenM DabrowskaM PatelM J T StubbingtonS A TeichmannO StegleKerstin B MeyerPublished in: Genome biology (2019)
In conclusion, we present robust protocols for tissue preservation for up to 24 h prior to scRNA-seq analysis. This greatly facilitates the logistics of sample collection for Human Cell Atlas or clinical studies since it increases the time frames for sample processing.