Biological Evaluation of 8-Methoxy-2,5-dimethyl-5H-indolo[2,3-b] Quinoline as a Potential Antitumor Agent via PI3K/AKT/mTOR Signaling.
Yunhao MaHongmei ZhuXinrong JiangZhongkun ZhouYong ZhouYanan TianHao ZhangMengze SunLixue TuJuan LuYuqing NiuHuan-Xiang LiuYingqian LiuPeng ChenPublished in: International journal of molecular sciences (2023)
Chemotherapy is commonly used clinically to treat colorectal cancer, but it is usually prone to drug resistance, so novel drugs need to be developed continuously to treat colorectal cancer. Neocryptolepine derivatives have attracted a lot of attention because of their good cytotoxic activity; however, cytotoxicity studies on colorectal cancer cells are scarce. In this study, the cytotoxicity of 8-methoxy-2,5-dimethyl-5H-indolo[2,3-b] quinoline (MMNC) in colorectal cells was evaluated. The results showed that MMNC inhibits the proliferation of HCT116 and Caco-2 cells, blocks the cell cycle in the G2/M phase, decreases the cell mitochondrial membrane potential and induces apoptosis. In addition, the results of western blot experiments suggest that MMNC exerts cytotoxicity by inhibiting the expression of PI3K/AKT/mTOR signaling pathway-related proteins. Based on these results, MMNC is a promising lead compound for anticancer activity in the treatment of human colorectal cancer.
Keyphrases
- signaling pathway
- induced apoptosis
- cell cycle
- cell cycle arrest
- pi k akt
- cell proliferation
- endothelial cells
- oxidative stress
- poor prognosis
- cell death
- epithelial mesenchymal transition
- single cell
- squamous cell carcinoma
- mesenchymal stem cells
- radiation therapy
- locally advanced
- bone marrow
- human health
- rectal cancer
- combination therapy
- long non coding rna