Genotypic detection of metronidazole and clarithromycin resistance in dyspeptic patients with helicobacter pylori.
Nader NemrNoha M Abd El-FadealAyman SalemMohamed O AbdallaNoha M Abd El-FadealNashaat M SolimanPublished in: Environmental science and pollution research international (2022)
In Egypt, antibiotic sensitivity analysis for Helicobacter pylori is not routinely performed. We aimed to identify the clarithromycin and metronidazole resistance directly from gastric biopsies for better guide treatment regimens. This cross-sectional descriptive study included 75 adult dyspeptic patients referred to the upper endoscopy unit in Suez Canal University Hospital, Ismailia, Egypt. Gastric biopsies were taken for rapid urease test (RUT) and cultured on brucella agar with antibiotic supplements. Genomic DNA was extracted directly from the specimen, and PCR was performed for direct detection of H. pylori. Also, to explore clarithromycin and metronidazole resistance, mutations in the 23S rRNA gene and the rdxA gene were investigated. We found that 60 samples were positive to RUT (80%), and only 4 samples were positive by culture. UreC gene was detected in 45 specimens. Meanwhile, 26 isolates were contained mutations at positions 2142 and 2143. Amplification of the metronidazole rdx gene was performed by conventional PCR. Out of 45 isolates, DNA sequence analysis of PCR product showed the wild type (ACA) in 9 isolates, while the mutant type (ATA) was detected in 28 isolates. We found a significant proportion of clarithromycin and metronidazole resistance among H. pylori infected patients in our region.
Keyphrases
- helicobacter pylori
- helicobacter pylori infection
- copy number
- wild type
- real time pcr
- genome wide
- cross sectional
- genetic diversity
- genome wide identification
- loop mediated isothermal amplification
- end stage renal disease
- newly diagnosed
- ejection fraction
- circulating tumor
- chronic kidney disease
- label free
- nucleic acid
- gene expression
- dna methylation
- high resolution
- transcription factor
- endothelial cells
- mass spectrometry
- prognostic factors
- genome wide analysis
- combination therapy
- replacement therapy