Atomic-Resolution Structure of SARS-CoV-2 Nucleocapsid Protein N-Terminal Domain.
Sucharita SarkarBrent RungeRyan W RussellKumar Tekwani MovellanDaniel CaleroSomayeh ZeinalilathoriCaitlin M QuinnManman LuGuillermo CaleroAngela M GronenbornTatyana PolenovaPublished in: Journal of the American Chemical Society (2022)
The nucleocapsid (N) protein is one of the four structural proteins of the SARS-CoV-2 virus and plays a crucial role in viral genome organization and, hence, replication and pathogenicity. The N-terminal domain (N NTD ) binds to the genomic RNA and thus comprises a potential target for inhibitor and vaccine development. We determined the atomic-resolution structure of crystalline N NTD by integrating solid-state magic angle spinning (MAS) NMR and X-ray diffraction. Our combined approach provides atomic details of protein packing interfaces as well as information about flexible regions as the N- and C-termini and the functionally important RNA binding, β-hairpin loop. In addition, ultrafast (100 kHz) MAS 1 H-detected experiments permitted the assignment of side-chain proton chemical shifts not available by other means. The present structure offers guidance for designing therapeutic interventions against the SARS-CoV-2 infection.
Keyphrases
- solid state
- sars cov
- respiratory syndrome coronavirus
- electron microscopy
- binding protein
- protein protein
- amino acid
- physical activity
- coronavirus disease
- healthcare
- risk assessment
- magnetic resonance imaging
- gene expression
- single molecule
- high frequency
- mass spectrometry
- dna methylation
- staphylococcus aureus
- climate change
- genome wide
- copy number
- nucleic acid
- cystic fibrosis
- dual energy
- energy transfer