abkBA toxin-antitoxin system may act as antipersister modules in Acinetobacter baumannii clinical isolates.
Fatemeh LatifiAli HashemiBehrooz S KalaniIraj PakzadAli HematianPublished in: Future microbiology (2023)
Aim: Persistence cells comprise a subpopulation of bacteria that is resistant to treatment. In this study, the role of the toxin-antitoxin (TA) system in the formation of persistence cells of Acinetobacter baumannii isolates was investigated. Methods: After confirming all isolates, TA systems abkBA, mqsRA and higBA were identified. Persister cells were confirmed using the standard method. Real-time PCR was used to compare the expression of TA systems in isolates in persistence and normal states. Results: The abkAB system was present in all isolates; 4% of isolates formed persister cells. The expression level of the abkB gene in persistent isolates showed a sevenfold increase compared with nonpersistent isolates. Conclusion: The abkBA system is proposed as an antipersistence target in A. baumannii isolates.
Keyphrases
- induced apoptosis
- acinetobacter baumannii
- genetic diversity
- cell cycle arrest
- multidrug resistant
- drug resistant
- pseudomonas aeruginosa
- poor prognosis
- escherichia coli
- endoplasmic reticulum stress
- signaling pathway
- gene expression
- cell death
- cell proliferation
- mass spectrometry
- long non coding rna
- high speed
- smoking cessation
- network analysis