Dual-Layered Approach of Ovine Collagen-Gelatin/Cellulose Hybrid Biomatrix Containing Graphene Oxide-Silver Nanoparticles for Cutaneous Wound Healing: Fabrication, Physicochemical, Cytotoxicity and Antibacterial Characterisation.
Atiqah SallehNorlaila MustafaYeit Haan TeowFatimah Mohd NorFauzul Azim KhairudinIshak AhmadMohd Fauzi Bin Mh BusraPublished in: Biomedicines (2022)
Tissue engineering products have grown rapidly as an alternative solution available for chronic wound and burn treatment. However, some drawbacks include additional procedures and a lack of antibacterial properties that can impair wound healing, which are issues that need to be tackled effectively for better wound recovery. This study aimed to develop a functionalized dual-layered hybrid biomatrix composed of collagen sponge (bottom layer) to facilitate cell proliferation and adhesion and gelatin/cellulose hydrogel (outer layer) incorporated with graphene oxide and silver nanoparticles (GC-GO/AgNP) to prevent possible external infections post-implantation. The bilayer hybrid scaffold was crosslinked with 0.1% ( w / v ) genipin for 6 h followed by advanced freeze-drying technology. Various characterisation parameters were employed to investigate the microstructure, biodegradability, surface wettability, nanoparticles antibacterial activity, mechanical strength, and biocompatibility of the bilayer bioscaffold towards human skin cells. The bilayer bioscaffold exhibited favourable results for wound healing applications as it demonstrated good water uptake (1702.12 ± 161.11%), slow rate of biodegradation (0.13 ± 0.12 mg/h), and reasonable water vapour transmission rate (800.00 ± 65.85 gm -2 h -1 ) due to its porosity (84.83 ± 4.48%). The biomatrix was also found to possess hydrophobic properties (48.97 ± 3.68°), ideal for cell attachment and high mechanical strength. Moreover, the hybrid GO-AgNP promoted antibacterial properties via the disk diffusion method. Finally, biomatrix unravelled good cellular compatibility with human dermal fibroblasts (>90%). Therefore, the fabricated bilayer scaffold could be a potential candidate for skin wound healing application.
Keyphrases
- wound healing
- silver nanoparticles
- tissue engineering
- cell proliferation
- endothelial cells
- single cell
- multiple sclerosis
- stem cells
- white matter
- highly efficient
- hyaluronic acid
- cell cycle arrest
- oxidative stress
- bone marrow
- cell death
- quantum dots
- cell cycle
- mass spectrometry
- staphylococcus aureus
- mesenchymal stem cells
- combination therapy
- pseudomonas aeruginosa
- gold nanoparticles
- drug delivery
- anti inflammatory
- pluripotent stem cells