1. The alterations of relative telomere length and expression of shelterin genes ( TRF1, TRF2, RAP1, POT1 , and TPP1 ) were evaluated from the chickens' right heart ventricle in the early and last stages of cold-induced pulmonary hypertension (PHS) at 21 and 42 d of age.2. The relative telomere length in the right ventricular tissues was significantly shorter in the PHS group of broilers than in the control group at 42 d, but did not statistically change at 21 d of age. There was a significant negative correlation between relative telomere length and RV:TV ratio in the broilers at 42 d of age.3. The relative expression of POT1 , RAP1 and TPP1 genes in the right ventricular tissues was significantly lower in the PHS group than in the control group at 21 d. The relative expression of the TRF2 gene was only higher in the PHS group of broilers than control at 42 d. The mRNA level of the TRF2 gene exhibited a significant positive correlation with RV:TV ratio at 42 d.4. It was concluded that most shelterin genes are dysregulated in the early stage of PHS (right ventricular hypertrophy) while telomere attrition occurs only at the last stage (heart dilation/failure).
Keyphrases
- poor prognosis
- genome wide
- pulmonary hypertension
- genome wide identification
- heat stress
- early stage
- mycobacterium tuberculosis
- binding protein
- gene expression
- heart failure
- bioinformatics analysis
- genome wide analysis
- blood pressure
- dna methylation
- copy number
- atrial fibrillation
- long non coding rna
- pulmonary arterial hypertension
- coronary artery
- lymph node
- high glucose
- sentinel lymph node
- drug induced