Xianfanghuomingyin, a Chinese Compound Medicine, Modulates the Proliferation and Differentiation of T Lymphocyte in a Collagen-Induced Arthritis Mouse Model.
Bo NieXue LiYi WeiMeng ChenJingwei ZhouLixia LouBin DongAi-Ming WuDongmei ZhangLingqun ZhuJiuli ZhaoLimin ChaiPublished in: Evidence-based complementary and alternative medicine : eCAM (2016)
In traditional Chinese medicine (TCM), xianfanghuomingyin (XFHM) is used to treat autoimmune diseases, including rheumatoid arthritis (RA). Here, we studied the mechanisms underlying its treatment effects, especially its anti-inflammatory effects in a collagen-induced arthritis (CIA) mouse model. We found that cartilage destruction and pannus formation were alleviated by treatment with XFHM. The abnormal differentiation of Th1 and Th17 cells was downregulated significantly by XFHM, and Th2 and Treg cells were upregulated. Moreover, the expression levels of specific cytokines and transcription factors related to Th1 cells (interferon γ [IFNγ], T-bet) and Th17 cells (interleukin- [IL-] 17) and the nuclear receptor retinoic acid receptor-related orphan receptor-gamma (RORγ) were downregulated. Serum IL-4 and GATA-3, which contribute to Th2 cells differentiation, increased significantly after XFHM administration. These results indicate that XFHM can restore the balance of T lymphocytes and reestablish the immunological tolerance to inhibit autoinflammatory disorder of RA. Taken together, XFHM can be used as a complementary or alternative traditional medicine to treat RA.
Keyphrases
- rheumatoid arthritis
- induced apoptosis
- cell cycle arrest
- mouse model
- signaling pathway
- disease activity
- oxidative stress
- transcription factor
- ankylosing spondylitis
- immune response
- cell death
- cell proliferation
- poor prognosis
- diabetic rats
- drug induced
- high glucose
- systemic sclerosis
- extracellular matrix
- long non coding rna
- stress induced