Antidiabetic and Antioxidative Potential of the Blue Congo Variety of Purple Potato Extract in Streptozotocin-Induced Diabetic Rats.
Paulina StrugałaOlha DzydzanIryna BrodyakAlicja Zofia KucharskaPiotr KuropkaMariana LiutaKatarzyna Kaleta-KuratewiczAgnieszka PrzewodowskaDorota MichałowskaJanina GabrielskaNatalia SybirnaPublished in: Molecules (Basel, Switzerland) (2019)
This study was designed to evaluate the effects of purple potato extract of the Blue Congo variety (PP) on diabetes and its antioxidant activities after two-week administration tostreptozotocin (STZ)-induced diabetic rats. The activities of PP were evaluated at a dose of 165 mg/kg body weight (b.w.) by estimating biochemical changes in blood plasma and through a histopathological study of kidney, muscles, and liver tissue. We evaluated the effect of treatment with extract on glucose level, glycated hemoglobin, activities of enzymatic antioxidants (including superoxide dismutase, glutathione peroxidase, and catalase), and lipid peroxidation. Moreover, we determined advanced glycation end-products (AGEs), advanced oxidation protein products (AOPPs), and the level of oxidative modified proteins (OMPs) as markers of carbonyl-oxidative stress in rats with diabetes. Using high-performance liquid chromatography, we identified five anthocyanins and six phenolic acids in the extract from Blue Congo with the dominant acylated anthocyanin as petunidin-3-p-coumaroyl-rutinoside-5-glucoside. The administration of Blue Congo extract lowered blood glucose, improved glucose tolerance, and decreased the amount of glycated hemoglobin. Furthermore, PP demonstrated an antioxidative effect, suppressed malondialdehyde levels, and restored antioxidant enzyme activities in diabetic rats. After administration of PP, we also noticed inhibition of OMP, AGE, and AOPP formation in the rats' blood plasma.
Keyphrases
- diabetic rats
- oxidative stress
- blood glucose
- anti inflammatory
- ischemia reperfusion injury
- induced apoptosis
- dna damage
- high performance liquid chromatography
- hydrogen peroxide
- body weight
- type diabetes
- glycemic control
- cardiovascular disease
- mass spectrometry
- clinical trial
- blood pressure
- nitric oxide
- heat shock
- tandem mass spectrometry
- adipose tissue
- insulin resistance
- solid phase extraction
- red blood cell
- simultaneous determination
- fatty acid
- protein protein
- replacement therapy
- liquid chromatography