Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture.

Timothy S UphoffBlake W BuchanNathan A LedeboerPaul A GranatoJudy A DalyTara N Marti

Published in: Journal of clinical microbiology (2016)

We compared group A Streptococcus (GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, the HDA method provided rapid, sensitive GAS detection, making culture confirmation unnecessary.

Keyphrases

histone deacetylase
loop mediated isothermal amplification
room temperature
candida albicans
biofilm formation
high throughput
cross sectional
ionic liquid
structural basis