Chromosomally integrated human herpesvirus 6 in the Japanese population.
Hiroki MiuraYoshiki KawamuraFumihiko HattoriKei KozawaMasaru IhiraTamae OhyeHiroki KurahashiTetsushi YoshikawaPublished in: Journal of medical virology (2018)
The objectives of the work are to elucidate the incidence and virological findings of chromosomally integrated human herpesvirus 6 (ciHHV-6) in Japanese population and to analyze an association between ciHHV-6 and the clinical manifestation of exanthema subitum (ES). Real-time polymerase chain reaction was performed to determine HHV-6 DNA loads in 2347 cord blood samples from healthy neonates (cohort A), febrile children less than 5 years old (cohort B), and hematopoietic cell transplant recipients (cohort C). CiHHV-6 was confirmed by detection of high copy numbers of viral DNA in somatic cells. The integration site was determined by fluorescent in situ hybridization analysis. In the ciHHV-6 subjects of cohorts A and B, HHV-6 antibody titers were measured, the history of ES was obtained, and the incidence of ES was compared with non-ciHHV-6 children without primary HHV-6B infection in the cohort B. CiHHV-6 was detected in 14 (0.60%) of the 2347 samples: A (6/1006, 0.60%), B (6/790, 0.76%), and C (2/551, 0.36%). The integration sites were on chromosome 22q in seven cases, Yp in two cases, and 17q and Xp in one case. No past history of ES was observed in 11 of the 12 subjects. Nine children with ciHHV-6 underwent serological analysis and were found to be positive for HHV-6 IgG antibodies. Incidence of ES was statistically higher in the control subjects than the ciHHV-6 subjects (P = 0.0039). In Japan, the frequency of ciHHV-6 was 0.60%. A high incidence of ciHHV-6A, specifically in chromosome 22, is a characteristic finding among the Japanese. CiHHV-6 may interfere with the clinical symptoms of primary HHV-6B infection.
Keyphrases
- risk factors
- cord blood
- endothelial cells
- young adults
- copy number
- induced apoptosis
- circulating tumor
- cell free
- induced pluripotent stem cells
- single molecule
- single cell
- bone marrow
- stem cells
- pluripotent stem cells
- oxidative stress
- gene expression
- cell proliferation
- mesenchymal stem cells
- cell cycle arrest
- sleep quality
- label free
- genome wide
- endoplasmic reticulum stress
- cell death
- hiv infected patients
- data analysis
- circulating tumor cells