Cryostabilization of the Cell Membrane of a Psychrotolerant Bacteria via Homeoviscous Adaptation.

Homeoviscous adaptation (maintenance of a critical balance between the saturated and unsaturated lipids) of the cell membrane of psychrotolerant bacteria is essential to protect them against freeze-thaw cycle. But how does the homeoviscous adaptation protect the cell membrane during cold stress? In this Letter, we answer this question using a coarse-grained molecular dynamics simulation technique. On the basis of the reported fatty acid profiles of psychrotolerant bacteria at different temperatures, multiple lipid membranes are simulated at a wide range of temperatures between 250 and 300 K. We explicate how the homeoviscous adaptation minimizes the effect of cold stress on the structure and fluidity of the membrane. Partial freezing of the saturated lipid domain occurs with the self-aggregation of saturated and unsaturated lipids near the melting temperature of the unadapted lipid membrane. The gel-like phase provides necessary local packing density that can be sensed by sensor proteins responsible for the homeoviscous adaptation.