Food-Grade Microscale Dispersion Enhances UV Stability and Antimicrobial Activity of a Model Bacteriophage (T7) for Reducing Bacterial Contamination (Escherichia coli) on the Plant Surface.

To reduce the use of conventional chemical pesticides, naturally occurring biopesticides such as bacteriophages have emerged as a promising solution, but effectiveness of these biopesticides can be limited because of their UV and desiccation instability. This study developed a biopolymer formulation to improve the phage stability, enhance the antimicrobial activity of phages, and prevent bacterial contaminations on a leaf surface in the presence of UV-A. The mixture of microscale polydopamine (PDA) particles with whey protein isolate (WPI)-glycerol formulation was effective for enhancing the stability of T7 phages in spraying solution and on a model leaf surface during 4 h exposure to UV-A and 1 h exposure to the simulated sunlight, respectively. The T7 phages incorporated with the biopolymer formulation effectively improved the antimicrobial activity of phages, as exhibited by greater than 2.8 log reduction in model bacteria Escherichia coli BL21 and also illustrated by significant potential of this formulation to prevent bacterial contamination and colonization of the plant surface. In summary, this study illustrates that phages combined with a biopolymer formulation can be an effective approach for a field deployable biocontrol solution of bacterial contamination in the agricultural environment.