Preservation stress resistance of melanin deficient conidia from Paecilomyces variotii and Penicillium roqueforti mutants generated via CRISPR/Cas9 genome editing.
Sjoerd J SeeklesPepijn P P TeunisseMaarten PuntTom van den BruleJan DijksterhuisJos HoubrakenHan A B WöstenArthur F J RamPublished in: Fungal biology and biotechnology (2021)
Our findings show the successful use of CRISPR/Cas9 genome editing and its high efficiency in P. variotii and P. roqueforti in both wild-type strains as well as kusA- mutant background strains. Additionally, we observed that melanin deficient conidia of three food spoiling fungi were not altered in their heat resistance. However, melanin deficient conidia had increased sensitivity towards UV-C radiation.