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Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells.

Mridusmita SaikiaPhilip BurnhamSara H KeshavjeeMichael F Z WangMichael HeyangPablo Moral-LopezMeleana M HinchmanCharles G DankoJohn S L ParkerIwijn De Vlaminck
Published in: Nature methods (2018)
We describe droplet-assisted RNA targeting by single-cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.
Keyphrases
  • single cell
  • rna seq
  • induced apoptosis
  • high throughput
  • cell cycle arrest
  • endoplasmic reticulum stress
  • gene expression
  • drug delivery
  • stem cells
  • dna methylation
  • mesenchymal stem cells
  • genome wide
  • cell therapy