High-resolution single-cell transcriptomic survey of cardiomyocytes from patients with hypertrophic cardiomyopathy.
Jiansen LuJie RenJie LiuMinjie LuYueli CuiYuhan LiaoYuan ZhouYun GaoFu-Chou TangJizheng WangShuiyun WangLu WenLei SongPublished in: Cell proliferation (2023)
Hypertrophic cardiomyopathy (HCM) is a common inherited cardiovascular disease, which can cause heart failure and lead to death. In this study, we performed high-resolution single-cell RNA-sequencing of 2115 individual cardiomyocytes obtained from HCM patients and normal controls. Signature up- and down-regulated genes in HCM were identified by integrative analysis across 37 patients and 41 controls from our data and published human single-cell and single-nucleus RNA-seq datasets, which were further classified into gene modules by single-cell co-expression analysis. Using our high-resolution dataset, we also investigated the heterogeneity among individual cardiomyocytes and revealed five distinct clusters within HCM cardiomyocytes. Interestingly, we showed that some extracellular matrix (ECM) genes were up-regulated in the HCM cardiomyocytes, suggesting that they play a role in cardiac remodelling. Taken together, our study comprehensively profiled the transcriptomic programs of HCM cardiomyocytes and provided insights into molecular mechanisms underlying the pathogenesis of HCM.
Keyphrases
- single cell
- hypertrophic cardiomyopathy
- rna seq
- left ventricular
- high resolution
- high throughput
- heart failure
- extracellular matrix
- cardiovascular disease
- end stage renal disease
- ejection fraction
- newly diagnosed
- cardiac resynchronization therapy
- transcription factor
- prognostic factors
- public health
- endothelial cells
- mass spectrometry
- peritoneal dialysis
- gene expression
- chronic kidney disease
- randomized controlled trial
- tandem mass spectrometry
- cardiovascular events
- single molecule
- artificial intelligence
- deep learning
- electronic health record
- machine learning
- genome wide identification
- binding protein