New Approach to Non-Invasive Tumor Model Monitoring via Self-Assemble Iron Containing Protein Nanocompartments.
Anna N GabashviliMaria V EfremovaStepan Sergeevich VodopyanovNelly S ChmelyukVera V OdaViktoria A SarkisovaMaria K LeonovaAlevtina S SemkinaAnna V IvanovaMaxim A AbakumovPublished in: Nanomaterials (Basel, Switzerland) (2022)
According to the World Health Organization, breast cancer is the most common oncological disease worldwide. There are multiple animal models for different types of breast carcinoma, allowing the research of tumor growth, metastasis, and angiogenesis. When studying these processes, it is crucial to visualize cancer cells for a prolonged time via a non-invasive method, for example, magnetic resonance imaging (MRI). In this study, we establish a new genetically encoded material based on Quasibacillus thermotolerans ( Q.thermotolerans, Qt ) encapsulin, stably expressed in mouse 4T1 breast carcinoma cells. The label consists of a protein shell containing an enzyme called ferroxidase. When adding Fe 2+ , a ferroxidase oxidizes Fe 2+ to Fe 3+ , followed by iron oxide nanoparticles formation. Additionally, genes encoding mZip14 metal transporter, enhancing the iron transport, were inserted into the cells via lentiviral transduction. The expression of transgenic sequences does not affect cell viability, and the presence of magnetic nanoparticles formed inside encapsulins results in an increase in T2 relaxivity.
Keyphrases
- magnetic resonance imaging
- magnetic nanoparticles
- iron oxide nanoparticles
- contrast enhanced
- binding protein
- induced apoptosis
- protein protein
- metal organic framework
- poor prognosis
- amino acid
- computed tomography
- aqueous solution
- endothelial cells
- prostate cancer
- cell cycle arrest
- diffusion weighted imaging
- iron deficiency
- oxidative stress
- vascular endothelial growth factor
- radical prostatectomy
- drug induced
- signaling pathway
- cell proliferation
- young adults
- minimally invasive
- transcription factor
- wound healing