A thermoresponsive microfluidic system integrating a shape memory polymer-modified textile and a paper-based colorimetric sensor for the detection of glucose in human sweat.

Textile-based microfluidic analytical devices have demonstrated significant potentials in biomolecular detection; however, to date, they have not been integrated with a shape memory polymer to prepare a thermoresponsive device for human sweat analysis. Herein, a thermoresponsive textile/paper-based microfluidic analysis system was constructed by combining biocompatible polyurethane (PU), cotton fabric and a paper-based colorimetric sensor. The coating of PU endowed the textile with temperature-dependent shape memory capability and patterned the channels to guide the liquid transport. A paper-based colorimetric sensor was prepared via a layer-by-layer deposition method and coupled with a smartphone for the quantitative analysis of glucose concentration. The as-prepared thermoresponsive textile/paper-based microfluidic analysis system had the dynamic range of 50-600 μM and the detection limit of 13.49 μM. After being fixed in the inner collar of a shirt, the system demonstrated great capabilities for the thermal-triggered sweat transport and in situ detection of glucose in human sweat under a high-temperature condition (59 °C). This study not only provides a low-cost and easy-to-wear sweat analysis tool for the health monitoring of people working at high temperatures, but also expands the applications of shape memory polymers and textile-based microfluidic devices in point-of-care testing.