The EspN transcription factor is an infection-dependent regulator of the ESX-1 system in M. marinum .
Kathleen R NicholsonRachel M CroninAruna R MenonMadeleine K JennischDavid M TobinPatricia A ChampionPublished in: bioRxiv : the preprint server for biology (2023)
Pathogenic mycobacteria cause acute and long-term diseases, including human tuberculosis. The ESX-1 system transports proteins that control the host response to infection and promotes bacterial survival. Although ESX-1 transports proteins, it also controls gene expression in the bacteria. In this study, we identify an undescribed transcription factor that controls the expression of ESX-1 genes, and is required for both macrophage and animal infection. However, this transcription factor is not the primary regulator of ESX-1 genes under standard laboratory conditions. These findings identify a critical transcription factor that controls expression of a major virulence pathway during infection, but whose effect is not detectable with standard laboratory strains and growth conditions.
Keyphrases
- transcription factor
- genome wide identification
- gene expression
- dna binding
- poor prognosis
- escherichia coli
- endothelial cells
- genome wide
- pseudomonas aeruginosa
- adipose tissue
- dna methylation
- mycobacterium tuberculosis
- emergency department
- staphylococcus aureus
- liver failure
- binding protein
- intensive care unit
- long non coding rna
- acute respiratory distress syndrome
- antimicrobial resistance
- respiratory failure
- hiv infected
- extracorporeal membrane oxygenation
- free survival
- mechanical ventilation